Young Hall 5060 & 5048 - UC Los Angeles Department of Chemistry & Biochemistry


Quantitative PCR

The CFX Connect qPCR system enables real-time detection of DNA copies amplified during PCR through the use of intercalating or primer based fluorophores. Quantitative PCR provides sensitive measurements of gene transcription levels, detects gene mutations down to the single base level, among many more nucleic acid probe based experiments; it can also be applied to protein stability assays using the Sypro-Orange or similar dyes.  The samples are measured with a trio of excitation LEDs and filtered emission detectors covering the range of 450 to 580nm; comprising nearly all commonly used fluorescent dyes and probes, with a dynamic range of 10 orders of magnitude.


Image and data taken from manufacturer’s website.