Young Hall 5060 & 5048 - UC Los Angeles Department of Chemistry & Biochemistry

310-206-2205 bita@chem.ucla.edu

Isothermal Titration Calorimetry (Microcal itc-200)

OLYMPUS DIGITAL CAMERA

The Microcal iTC-200 is designed to make very precise calculations of the binding energetics of analyte molecules, most commonly a protein and ligand.  Through precise measurements of enthalpy changes produced by titration of one analyte into the other, this instrument can calculate binding constants and stoichiometry with a high degree of accuracy.  This machine uses a far smaller sample volume than previous models (only 200 μL), opening this instrument to experiments with analytes that are difficult to produce in quantity. Please note that the design of this instrument was focused on biochemical/aqueous applications, so many organic solvents are incompatible with the iTC-200 (DMSO is acceptable).

iTC_dataData and  figures taken from Instrument manufacturers website.