Bio-layer interferometry uses the interference produced from two light reflections of a single source to measure the aggregation of a target molecule on the sensor surface: as the target molecules aggregate or dissociate from the probe surface, the distance of between the reflections sources change accordingly. Simple dip and read measurements can be used to identify the presence of a molecule in a solution, while time-course analysis allows for measurements of binding affinities. A wide range of probes are available (standard antibodies, Protein A, Ni-NTA, Streptavidin, etc.) making this system extremely flexible in application; furthermore, limited custom probe production is possible in coordination with the manufacturer. For information on purchasing tips or having custom tips created, visit the instrument manufacturer’s website: ForteBio BLItz.
Image and data taken from manufacturer’s website